Journal: Cell reports

Article Title: Defective extracellular matrix remodeling in brown adipose tissue is associated with fibro-inflammation and reduced diet-induced thermogenesis.

doi: 10.1016/j.celrep.2023.112640

Figure Lengend Snippet: Figure 1. Long-term HFD exposure induces BAT fibro-inflammation (A and B) Heatmap of metabolic parameters (including glycemia [gly], triglycerides [TG], and insulinemia [ins]) (A) and mRNA expression of metabolic and fibro- inflammatory marker (B) from C57Bl/6 mice fed 2, 8, 16, or 28 weeks (n = 8/group) with chow diet (Ch) or HFD 45% (HF).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-collagen I Abcam Cat# ab21286; RRID:AB_446161 Anti-collagen III Abcam Cat# ab7778; RRID:AB_306066 Anti-collagen IV Abcam Cat# ab6586; RRID:AB_305584 Anti-collagen V Abcam Cat# ab7046, RRID:AB_305723 Anti-collagen VI Abcam Cat# ab182744, RRID:AB_284791 Anti-fibronectin Abcam Cat# ab2413, RRID:AB_2262874 MACS mouse CD11b microbeads Miltnyibiotec Cat# 130-049-601, RRID:N/A MACS mouse CD45 microbeads Miltnyibiotec Cat# 130-052-301, RRID:N/A MACS mouse CD31 microbeads Miltnyibiotec Cat# 130-097-418, RRID:N/A Alexa 555-conjugated anti-rabbit Life Technologies Cat# A-21428, RRID:AB_2535849 Alexa 488-conjugated anti-mouse Life technologies Cat# A-21202, RRID:AB_141607 Chemicals, peptides, and recombinant proteins Lipopolysaccharide Sigma-aldrich Cat# L4391 (±)-Norepinephrine (+)-bitartrate salt Sigma Cat# A0937-5G Insulin (Actrapid 100 IU/mL) Novo Nordisk Cat# 1331415 Bodipy 493/503 Life science Cat# D-3922 Phalloidin-iFluor 594 Reagent Abcam Cat# ab176757 Hoechst 33342 Thermofisher Cat# H3570 PuraMatrix hydrogel Corning Life Science Cat# 354250 45% HFD 58% HFD Research Diets Cat# D12451 Cat# 12331 Triton X-100 Sigma-aldrich Cat# T-9284 STAT60 AMS Biotechnology Cat# CS-502 Collagenase from Clostridium histolyticum type 2 Sigma-aldrich Cat# C6885 Bovine serum albumin Sigma-aldrich Cat# A8412 Critical commercial assays Free glycerol assay Sigma-aldrich Cat# F6428-40mL RNeasy minikit (250) Qiagen Cat# 74106 RNA 6000 Nano Kit Agilent Cat# 5067–1511 TruSeq Stranded mRNA HT Sample Prep Kit Illumina Cat# 20020595 Proteome Profiler Mouse Angiogenesis Array Kit R&D Systems Cat# ARY015 Deposited data Single nuclei RNAseq: murine BAT Y Wang et al.35 GEO: GSE202630 Single nuclei RNAseq: human BAT W Sun et al.36 EBI: E-MTAB-8564 RNAseq data: human NE-treated adipocytes KV Tran et al.46 N/A RNAseq data: PAZ6 adipocytes SC and ACG et al.62 GEO: GSE168387 RNAseq data: mice exposed to cold and TN J Sanchez-Gurmaches et al.47 GEO: GSE96681 Human RNAseq data M U-Din et al.42 GEO: GSE113764 Experimental models: Cell lines Murine immortalised brown preadipocyte cells (C57BAT).

Techniques: Expressing, Marker